Piperine Alkaloid Induces Anti-Cancer Effects in Human Breast Carcinoma Cells by Targeting Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway

Piperine is a dietary alkaloid with huge pharmacological importance and was first isolated from pepper extract. It has been reported anti-cancer, anti-amoebic, anti-mycobacterial, anti-mutagenic, anticonvulsant, anti-asthmatic, immunomodulatory, anti-inflammatory anti-oxidant activity. Therefore, the current investigation designed to unveil anticancer effects of piperine molecule against human breast carcinoma. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay executed for determination on cancer cell viability. Clonogenic implemented checking effect clonogenic potency cells. To monitor morphology cancerous cells, phase contrast microscopy performed after exposure. Apoptosis checked by acridine orange/ethidium bromine staining execution Western blotting analysis. Cell migration invasion monitored transwell chambers assay. Muse flow cytometric analysis assessing different cycle phases. check levels phosphatidylinositol 3-kinase protein kinase B. Results revealed that induced dose reliant cytotoxicity in MCF-7 Further, application colonies remarkably reduced number minimum. Phase evidenced some specific morphological modifications indicating apoptosis allied like apoptotic bodies, condensed nucleus membrane blabbing. Acridine cells which further investigated blotting. This increased expression B-cell lymphoma 2 associated X 2, induction piperine. as well invasive ability minimum dependent-manner. Flow arrested at growth 2/mitosis phase, hence suppressed progression. Finally, predicted constant B phosphorylated 3-kinase. Hence, blocking 3-kinase/protein signaling pathway. In conclusion, regarding remarkable suppression apoptosis, invasion, blocked 3-kinase/